F7252
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F7252
Sigma
Fast Green FCF
Dye content ~90 %
| Synonym: | Food green 3 |
| CAS Number: | 2353-45-9 |
| Linear Formula: | C37H34N2O10S3Na2 |
| Molecular Weight: | 808.85 |
| Colour Index Number: | 42053 |
| Beilstein Registry Number: | 5718212 |
| EC Number: | 219-091-5 |
| MDL number: | MFCD00013053 |
| PubChem Substance ID: | 24894934 |
Description
| Application | Fast Green FCF dye is used for protein staining in IEF, native PAGE and SDS-PAGE. Fast Green staining is linear over a wider range of protein concentrations than Brilliant Blue R. After electrophoresis, fixing the proteins in the gel is recommended for maximum sensitivity. The gel can then be stained for 2 hr with 0.1% Fast Green FCF in either 30% (v/v) ethanol, 10% (v/v) acetic acid or in 7% acetic acid. The gel can be destained with either 30% (v/v) ethanol, 10% (v/v) acetic acid, or 7% acetic acid. The stained gel can be scanned at 625 nm. The detection sensitivity is approx. 30% that with Brilliant Blue R. |
Properties
| composition | Dye content, ~90% |
| mp | 290 °C (dec.)(lit.) |
Safety
| Personal Protective Equipment | dust mask type N95 (US), Eyeshields, Gloves |
| Hazard Codes | Xn |
| Risk Statements | 68 |
| Safety Statements | 36/37 |
| WGK Germany | 2 |
| RTECS | BQ4425000 |
References
| reference | Allen, R.E., et al., Staining protein in isoelectric focusing gels with fast green. Anal. Biochem. 104, 494-498, (1980) |
| Wilson, C.M., Staining of proteins on gels: comparisons of dyes and procedures. Meth. Enzymol. 91, 236-247, (1983) | |
| Merck | Merck 13,3970 |
| Beilstein | Beil. 16,II,513 |
| Aldrich MSDS 1, 945:D / Corp MSDS 1 (1), 1678:A / FT-IR 1 (2), 1033:C / FT-IR 2 (3), 4134:B / IR-Spectra (3), 1485:D / RegBook 1 (2), 2779:I / Sax 6, 1411 / Sigma FT-IR 1 (2), 141:B / Stains and Dyes Ref, 345 / Structure Index 1, 442:B:2 |
